WebpsiCHECK-2 Description psiCHEC-2 Vector is designed to provide a quantitative and rapid approach for optimization of RNA interference (RNAi). The vectors enable the monitoring of changes in expression of a target gene fused to the reporter gene. WebDec 18, 2024 · Wild-type or mutant circCHST15 or 3′-UTR EIF4EBP1 was synthesized and then subcloned into psiCHECK-2 vector. HEK293T cells were seeded in 24-well plates at a concentration of 1.5 × 105 per well and cotransfected miR-125a-5p mimics or miR-NC with psiCHECK-2-wt-circCHST15, psiCHECK-2-mut-circCHST15, psiCHECK-2-wt-EIF4EBP1 or …
circCHST15 is a novel prognostic biomarker that promotes clear …
WebThe psiCHECK™-2 Vector contains a second reporter gene, a synthetic firefly luciferase gene (hluc+), and is designed for endpoint lytic assays. Renillaand firefly luciferase activity can be measured using either the Dual-Luciferase® Reporter Assay System(c,g,l,m) (Cat.# E1910) or Dual-Glo™ Luciferase Assay System(c,g,l,m) (Cat.# E2920 ... WebMCF-7 cells were transfected with a reporter vector psiCHECK2-FSCN1 3'UTR or psiCHECK2 control vector and miR-133a mimic or negative control. Each transfection was carried out in triplicate. Luciferase assay was performed 48 h after gene transfection. Firefly luciferase activity was normalized to Renilla luciferase activity. par-berlin.com
Post-transcriptional regulation DPC4 gene by miR-190 in
WebThe psiCHECK-2 Vector is a dual Luciferase vector designed to provide a quantitative and rapid approach for initial optimization of RNA interference (RNAi). The vector enable monitoring of changes in expression of a target gene fused to a reporter gene. The Renilla luciferase is used as the primary reporter gene, and the gene of interest is ... WebLuciferase Reporter Vector Psicheck 2, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Average 86 stars, based on … WebThe regulation of DPC4 by miR-190 was evaluated by qRT-PCR, Western blotting, and luciferase reporter assays in the human CRC cell line HT-29 after treatment with miR-190 mimics and inhibitors. Results: The DPC4 mRNA, miR-, and DPC4 protein expression levels were highest in LS174T cells while lowest in SW480 and SW620 cells. parber shop west palm beach rikki freer