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Psicheck-2 vector

WebpsiCHECK-2 Description psiCHEC-2 Vector is designed to provide a quantitative and rapid approach for optimization of RNA interference (RNAi). The vectors enable the monitoring of changes in expression of a target gene fused to the reporter gene. WebDec 18, 2024 · Wild-type or mutant circCHST15 or 3′-UTR EIF4EBP1 was synthesized and then subcloned into psiCHECK-2 vector. HEK293T cells were seeded in 24-well plates at a concentration of 1.5 × 105 per well and cotransfected miR-125a-5p mimics or miR-NC with psiCHECK-2-wt-circCHST15, psiCHECK-2-mut-circCHST15, psiCHECK-2-wt-EIF4EBP1 or …

circCHST15 is a novel prognostic biomarker that promotes clear …

WebThe psiCHECK™-2 Vector contains a second reporter gene, a synthetic firefly luciferase gene (hluc+), and is designed for endpoint lytic assays. Renillaand firefly luciferase activity can be measured using either the Dual-Luciferase® Reporter Assay System(c,g,l,m) (Cat.# E1910) or Dual-Glo™ Luciferase Assay System(c,g,l,m) (Cat.# E2920 ... WebMCF-7 cells were transfected with a reporter vector psiCHECK2-FSCN1 3'UTR or psiCHECK2 control vector and miR-133a mimic or negative control. Each transfection was carried out in triplicate. Luciferase assay was performed 48 h after gene transfection. Firefly luciferase activity was normalized to Renilla luciferase activity. par-berlin.com https://goboatr.com

Post-transcriptional regulation DPC4 gene by miR-190 in

WebThe psiCHECK-2 Vector is a dual Luciferase vector designed to provide a quantitative and rapid approach for initial optimization of RNA interference (RNAi). The vector enable monitoring of changes in expression of a target gene fused to a reporter gene. The Renilla luciferase is used as the primary reporter gene, and the gene of interest is ... WebLuciferase Reporter Vector Psicheck 2, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Average 86 stars, based on … WebThe regulation of DPC4 by miR-190 was evaluated by qRT-PCR, Western blotting, and luciferase reporter assays in the human CRC cell line HT-29 after treatment with miR-190 mimics and inhibitors. Results: The DPC4 mRNA, miR-, and DPC4 protein expression levels were highest in LS174T cells while lowest in SW480 and SW620 cells. parber shop west palm beach rikki freer

psiCHECK™-2 Vector RNAi Assay - Promega

Category:Psicheck2 Dual Luciferase Reporter Vector Promega Bioz

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Psicheck-2 vector

siCHECK(TM) Vectors Technical Bulletin TB329 - Promega

WebpsiCHECK2 (Search Vector Database) Backbone manufacturer Promega Backbone size (bp) 6273 Modifications to backbone Insertion of a SalI/NotI/XhoI linker between the NotI and XhoI site Vector type Mammalian Expression Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number WebFeb 21, 2024 · In the psiCHECK-2 vector, Renilla is expressed under SV40_promotor/enchancer and is a test luciferase, whereas Firefly is expressed under …

Psicheck-2 vector

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WebMay 5, 2009 · UTR sequences, up to and including the primary ATG initiation codon, were synthesized (IDT), cloned and ligated into the NheI site directly preceding the Renilla luciferase gene in the dual-luciferase vector psiCHECK-2 (Promega) . WebFeb 16, 2024 · PsiCHECK-2 vector contained the ampicillin (Amp) resistance gene. The recombinant plasmids were obtained through transformation of Escherichia coli (E. coli) DH5a bacteria as a host.

WebPsicheck 2 Luciferase Reporter Vector, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … WebJul 7, 2024 · In this study, we replaced the SV40 promoter in the psiCHECK-2 reporter vector, which is widely used with mammalian cell lines, with the HSV-TK or AC5.1 …

WebpsiCHECK-2 (Search Vector Database) Backbone manufacturer Promega Backbone size w/o insert (bp) 6273 Vector type Luciferase Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number High Copy Gene/Insert Gene/Insert name 3 miR-122 target sites Insert Size (bp) 69 WebpsiCHECK-1 and psiCHECK-2 Vectors enable monitoring of changes in expression of a target gene fused to a reporter gene. They are used for optimizing RNA interference …

WebBy cloning the 3’UTRs of collagen IV subunits into the psiCHECK TM-2 vector, we found that seven of the eight sites in the Col4a3 – Col4a6 gene complementary to miR-29 were …

WebThere are two siCHECK™ Vectors, the psiCHECK™-1 Vector and the psiCHECK™-2 Vector. Both vectors contain as the primary reporter gene the synthetic version of Renilla … parbhani sonpeth pin codeWebThe psiCHECK™-2 Vector is designed to provide quantitative and rapid optimization of RNA interference (RNAi). The vector enables monitoring of changes in expression of a target … timeshares outer banksWebpsiCHECK-1 and psiCHECK-2 Vectors enable monitoring of changes in expression of a target gene fused to a reporter gene. They are used for optimizing RNA interference … parberry bellingham waWebpsiCHECK-2 Description psiCHEC-2 Vector is designed to provide a quantitative and rapid approach for optimization of RNA interference (RNAi). The vectors enable the monitoring … time shares orange beach alabamaWebThe psiCHECK™-2 Vector, which contains two luminescent reporter genes, was used to identify the most effective shRNA for inducing RNAi of the bcr/abl gene. The seven shRNAs tested were generated using the T7 RiboMAX™ Express RNAi System. Introduction parb family transcriptional regulatorWebIn the psiCHECK-2 vector, Renilla is expressed under SV40_promotor/enchancer and is a test luciferase, whereas Firefly is expressed under HSV-TK promoter and is an endogenous control. Therefore,... timeshares on the beachparb government