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WebApr 21, 2024 · After washing the cells 3 times with pre-cooled PBS (pH 7.4), they were centrifuged at 5000× g for 5 min and we then discarded the supernatant. The cell pellets … WebScrape adherent cells off the dish using a cold plastic cell scraper, then gently transfer the cell suspension into a pre-cooled microcentrifuge tube. Alternatively cells can be … WebPreparation of protein samples. 1.Protein extraction. 1)For tissue sample a. Take the samples, wash the tissue thoroughly with pre-cooled PBS (0.01 M, pH=7.4)(Cat# E-BC-R187) to remove the surface blood and internal debris.b. Weigh and smash the tissue, add an appropriate ratio of RIPA Lysis Buffer (Cat# E-BC-R327)(add 10 μL PMSF and 10 μL Na 3 … indira college wakad