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Plko.1 – trc cloning vector

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August undefined, 2024

Webb10 sep. 2024 · The shRNA sequences were constructed into the pLKO.1-TRC cloning vector through EcoRI and AgeI sites to generate pLKO.1-shRNA recombinants (Lv-shINSIG1). Meanwhile, the INSIG1 gene open reading frame sequence was amplified and inserted into the vector through Xho I and Hand III sites to get pEGFP-INSIG1-N1 … WebbpLKO.1 价格： 1500元联系方式： I47-825O-882O 相关技术服务：质粒构建基因合成质粒大提立刻购买买家导航载体基本信息载体图谱质粒图谱和多克隆位点信息载体简介载体序列 LOCUS pLKO.1 8901 bp ds-DNA circular SYN 08-JAN-2014 DEFINITION Stuffer-containing lentiviral vector for expression of shRNA. This plasmid is the official TRC …

【工具】shRNA的设计 - 知乎

Webb本发明公开了抑制PHB基因的物质在胶质瘤治疗中的应用。本发明发现，PHB基因的敲除或敲低可显著降低GSC的肿瘤细胞球形成能力、细胞活力，并可显著降低GSC细胞的克隆形成能力，即可以显著抑制细胞的自我更新能力，但对普通的非干性肿瘤细胞的细胞活力无影响。本发明的实验还进一步证明，PHB ... WebbAddgene inc generation transfer plasmid plko 1 trc cloning vector Generation Transfer Plasmid Plko 1 Trc Cloning Vector, supplied by Addgene inc, used in various techniques. … tackker chrome store

TRC Lentiviral shRNA - Horizon Discovery

http://www.biofeng.com/zaiti/manbingdu/pLKO.1-TRC.html WebbpLKO.1 (Search Vector Database) Backbone size (bp) 7032 Vector type Mammalian Expression, Lentiviral, RNAi Selectable markers Puromycin Growth in Bacteria Bacterial … Webb博奥森金牌抗体——Beta-Actin Polyclonal Antibody (bs-0061r). Bioss经过16年的潜心抗体研发，开发了金牌内参抗体：Anti-beta-Actin antibody（bs-0061R）。该抗体具有灵敏度高、特异性强、交叉反应物种丰富、性价比高等优质特点，已被许多科研人员所认可，引用发表 … tackl cleveland hopkins

Cloning shRNA Oligos into pLKO.1 - protocols.io

Construction of Conveniently Screening pLKO.1-TRC Vector …

Webb11 feb. 2024 · Methods: The 1.9 kb insertion fragment of the original pLKO.1-TRC cloning vector was excised by digestion with Age I and EcoR I, and the annealing shRNA oligos of mSelK were cloned into the Age I and EcoR I sites to construct pLKO.1-mSelK-shRNA vector. Then the constructed pLKO.1-mSelK-shRNA vector, psPAX2 packaging plasmid … WebbThe MISSION ® TRC shRNAs are cloned into the pLKO.1 transfer vector that is compatible with standard 2 plasmid (packaging vector with rev gene and envelope vector) or 3 … tackl cleWebb3 juni 2016 · 先在体外构建能表达双链RNA的载体,再将载体转移到细胞内在细胞内合成出双链RNA,不但能增加有效转染细胞的种类,而且在长期稳定表达载体的细胞株中,双链RNA能够长期发挥阻断基因的作用。. 现在有多个报道说通过质粒表达siRNAs同样可以抑制特定基因 … tackk for education

"WebbThe pLKO.1-puro Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl. Application MISSION ® pLKO.1-puro Empty … " - Plko.1 – trc cloning vector

Plko.1 – trc cloning vector

http://www.biofeng.com/zaiti/manbingdu/pLKO.1.html WebbDoes pLKO.1 contain WPRE? No, the TRC1 vector, pLKO.1-puro, does not contain WPRE (Woodchuck Posttranscriptional Regulatory Element). It is contained in the TRC2 vector, …

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Webbcell lines (Fig. 1c and Supplementary File 1). We used the DAVID resource for automated annotation and functional clas-sification of the differentially expressed genes. Among the top biofunctions, we found protein metabolism, ribosomal Fig. 1 Transcriptional effect of RHBDD2-siRNA silencing in breast cancer cell lines. Webb11 okt. 2016 · The pLKO.1-TRC plasmid derived from pRRLSIN.cPPT.PGK/GFP/WPRE is a third-generation self-inactivating lentiviral vector. In this plasmid, a human U6 promoter is used to drive the expression of shRNA, and a PGK promoter is used to drive the expression of puromycin resistance gene for selecting the transduced cells [ 5 – 7 ].

WebbPreparation of pLKO.1 TRC for cloning . 1. Digest pLKO.1 TRC-cloning vector with AgeI and EcoRI. 2. Purify the 7 kb band by gel extraction. We used The illustra™ GFX™ PCR DNA and Gel. Band Purification Kit from GE Healthcare, but a gel extraction method of choice can be used. Quantify the DNA and proceed to ligation. WebbPlko 1 Trc Cloning Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

Webb12 juni 2024 · pcdna3.1-myc-hisB PLKO-TRC-cloning-vector pIRES2-EGFP pLVX-shRNA2 lenti MS2-P65-HSF1_Hygro addgene61426 lenti sgRNA(MS2)_puro backbone addgene73795 pcDNA3.1(+) CircRNA Mini Vector addgene60648 pCLucIPZ addgene53222 pGIPZ pJL-TRBO addgene80082 pLSLR addgene51500 pMA2775 addgene46884 WebbHere, the ‘stuffer’ sequence of the pLKO.1-TRC cloning vector was excised in exchange for a constructed shRNA insert targeting CDK12. Lentiviral particles were produced, carrying these modified pLKO.1- TRC plasmids, and transfected into the MDA-MB-231 breast cancer cell line in an effort to deplete the cells’ expression of CDK12.

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Webb1 feb. 2024 · The pLKO.1-TRC plasmid is a third-generation selfinactivating lentiviral vector, in which a human U6 promoter drives the shRNA expression while a PGK … tackl covid testing pittsburghWebbpLKO.1 - TRC cloning vector. Plasmid. #10878. Purpose. 3rd generation transfer plasmid. Depositor. David Root. Article. Moffat et al Cell. 2006 Mar 24. 124 (6):1283-98. tackl healthWebbA. pLKO.1-TRC Cloning Vector A.1 The RNAi Consortium The pLKO.1 cloning vector is the backbone upon which The RNAi Consortium has built a library of shRNAs directed against 15,000 human and 15,000 mouse genes. Addgene is working with the TRC to make this shRNA cloning vector available to the scientific community. tackl health cleveland airportWebbThe Genetic Perturbation Platform, formerly known as the RNA interference (RNAi) Platform, supports functional investigations of the mammalian genome that can reveal how genetic alterations lead to changes in phenotype. tackl covid testing grand rapidsWebbThe pLKO1 lentiviral vector enables efficient transduction of primary and non-dividing cells, such as neuronal cells making it easy to perform RNAi studies in these hard to transfect cell lines. Stable selection is also possible using the puromycin selectable marker. tackl covid test grand rapidsWebbFind the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David Root lab is a 3rd generation lentiviral transfer plasmid for cloning and expressing new shRNA sequences. For more information, see the Addgene’s pLKO.1 protocol. Pro-tip: this plasmid grows more slowly than standard plasmids. Find pLKO.1 - TRC cloning ... tackl covid testing savannahWebb9 jan. 2013 · Elute DNAconcentration. C.4 Ligating Useyour ligation method standardT4 ligation, mix: annealedoligo from step C.2. 20 ng digested pLKO.1 TRC-cloning vector from step C.3. youwere unable DNAconcentration, use 10xNEB T4 DNA ligase buffer NEBT4 DNA ligase 4-20hours. ligationmix competentDH5 alpha cells, following manufacturer’s … tackl health cleveland