Klh-conjugated linear peptide
WebAmino or COOH-conjugation chemistries should be avoided as most peptides contain several NH2 and COOH groups available in a given peptide sequence which can result in forming multiple attachment points or peptide distortion. 15. W h at i s a MAP ? MAPS or Multi-Antigenic Peptide is a branched peptide at which linear peptide chains are linked at ... WebAug 24, 2024 · We evaluated the immunogenicity, neutralization and protective efficacy of all RSV-G antigenic sites identified following primary RSV infection using recombinant E. coli expressed G ectodomain (REG), CCD-deleted G ectodomain (REG ΔCCD), N- and C-terminal G subdomains, and antigenic site peptides.
Klh-conjugated linear peptide
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WebProduct Description. Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein in the production of antibodies. Low molecular weight haptens such as peptides, … WebJun 29, 2024 · The KLH-conjugated peptide was then mixed with 100 μ L of complete Freund’s adjuvant (CFA, Cat. F5881, Sigma, USA) for intraperitoneal inoculation at week 0. Fifty micrograms of KLH-conjugated peptide and 50 μ L of incomplete Freund’s adjuvant (IFA, Cat. F5506, Sigma, USA) were mixed for reimmunization at weeks 2, 4, 5, 6, 7, and 8.
WebKLH is an effective carrier protein for several reasons. Its large size and numerous epitopes generate a substantial immune response, and abundance of lysine residues for coupling haptens allows a high … http://novitchlab.com/sites/default/files/Peptide%20conjugation%20to%20KLH.pdf
Web 第 6 年 经营模式：经销商 商铺产品：9983条 所在地区：上海上海市 注册时间：2024-04-17 最近登录：2024-04-14 WebConjugation is used to couple the antigenic peptide to either BSA or KLH. Because of the differences in the molecular weight of BSA and KLH, different calculations are used to …
WebConjugation: ALEXA FLUOR® 350: Host: Rabbit: Source: KLH conjugated synthetic peptide derived from human DGAT1: Immunogen Range: 251-350/488: Clonality: Polyclonal: Isotype: IgG: Concentration: 1ug/ul: Purification: Purified by Protein A. Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and ...
WebAs low as $3/aa or $90/peptide for custom peptide synthesis services. As low as $25/peptide for peptide library services. From crude to 99% purity; from milligrams to kilograms. Wide range of peptide modifications (250+) are available. Click Peptide Modifications tab below or download here. health teacher jobsWebFeb 1, 2006 · KLH-conjugated peptides (KLH peptides) were stored at -20°C until used. Immunochemical assay Unless otherwise indicated, all incubation steps were performed at 25°C. PBS, PBS containing 0.05% Tween 20 (PBS-T20), and PBS containing 0.5% BSA (PBS-BSA) were used as the coating, washing/dilution, and blocking buffers, respectively. health teacherWebHapten and peptide antigen conjugation services for immunogen preparation and antibody production We offer multiple options for conjugating peptide antigens or haptens to immunogenic carrier proteins using strategies that optimize antigen presentation and antibody titer for custom antibody production services. health teaWebDec 8, 1992 · KLH was first dissolved in buffers containing 3 M guanidine hydrochloride to maintain solubility and derivatized with either of two water soluble, heterobifunctional crosslinkers, m-maleimido-benzoyl-N-hydroxy-sulfosuccinimide ester (SMBS), or sulfosuccinimidyl 4- (N-maleimidomethyl)cyclohexane-1-carboxylate (SSMCC) (300:1 … healthteacher.comWebKLH-conjugated linear peptide corresponding to human PLAC3. Packaging 100 μg Application Anti-PLAC3 Antibody is a rabbit polyclonal antibody, validated for use in western blotting, ICC & IHC. Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected PLAC3 in human placenta and human breast cancer tissues. health teacher certificationWebJun 10, 2024 · KLH is a large aggregating protein and has a MW that ranges from 4*105 to 1*107 Da. One of the advantages of KLH is that it does not interfere with ELISA or western … good food pubs near hereWebKey Features of this KLH-Peptide Conjugation Kit: High quality maleimide-activated KLH for the conjugation: >95% purity High maleimide groups per KLH for peptide loading: 50-100 … health teacher jobs ct