Fitc annexin v凋亡检测试剂盒
Web原理. Sigma的Annexin V-Cy3试剂盒可以配合荧光显微镜来检测凋亡细胞上结合的膜联蛋白V。. 该试剂盒采用Cy3.18作为偶联膜联蛋白的荧光染料。. 通过显微镜检测发 … Web1. Wash cells twice with cold PBS and then resuspend cells in 1X Binding Buffer at a concentration of 1 x 10^6 cells/ml. 2. Transfer 100 µl of the solution (1 x 10^5 cells) to a 5 ml culture tube. 3. Add 5 µl of FITC Annexin V and 5 µl PI. 4. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark. 5.
Fitc annexin v凋亡检测试剂盒
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WebThe Annexin V-FITC Kit contains reagents for the simultaneous fluorescent detection of apoptotic, necrotic, and dead cells through the specific binding of FITC-labeled Annexin V to phosphotidylserine (PS). The kit consists of Annexin V-FITC, Annexin V Binding Buffer, and Propidium Iodide Solution. WebDead Cell Apoptosis Kit with Annexin V FITC and PI, for flow cytometry: Kit Contents: Contains 1 vial of annexin V, FITC conjugate (250 µL ), 1 vial of propidium iodide (PI, 100 µL), and 1 bottle of annexin binding buffer (5X …
WebGently mix and incubate at RT for 15 min in the dark. *For Annexin V-Biotin samples only: After 15 min incubation, wash once with 1 ml of 1X Binding Buffer. Dilute 0.5 µg Streptavidin-Fluorescein Isothiocynate (SAv-FITC, Cat. No. 554060) into 100 µl of 1X Binding Buffer and add to the cell pellet. Gently mix the cells and incubate at RT for ... WebAnnexin V is a Ca 2+-dependent phospholipid binding protein. This protein can bind to a variety of phospholipids, but it has the highest affinity for phosphatidylserine (PS). The ApoDETECT Annexin V-FITC Kit binds to …
Web下文是基于 Annexin V-FITC 细胞凋亡检测试剂盒 (ab14085) 提供的方案设计的使用 Annexin V 进行 PS 暴露检测的方案示例。 需要注意的是,当使用某一试剂盒时,请务必 … Web2. Prepare 1X Annexin-binding buffer. All Invitrogen Annexin V kits, come with Annexin V binding buffer. 3. Prepare a 100 µg/mL working solution of PI by diluting 5 µL of the 1 mg/mL PI stock solution in 45 µL 1X Annexin-binding buffer. Store the unused portion of this working solution for future experiments. 4.
Web2. Annexin-V染料上带FITC荧光,因此从染色开始注意避光操作和孵育,一般尽量在染色后半个小时内 上机检测。 3. 如果处理的细胞中含有GFP会干扰FITC的荧光信号,应该更换不同波长的荧光染料标记的Annexin-V。 4.
WebAnnexin V-FITC试剂盒可以荧光检测与annexin V结合的凋亡细胞,并使用流式细胞仪进行定量。AnnexinV-FITC试剂盒使用荧光素异硫氰酸酯(FITC)偶联的annexin V标记细 … md prevailing wage lawWeb使用用于进行流式细胞分析的 eBioscience 和 Annexin V 细胞凋亡检测试剂盒,可轻松区分早期和晚期细胞凋亡。. 这些试剂盒利用活力染料偶联膜联蛋白 V 标记细胞外膜上的磷 … mdp resume writing servicesWebAnnexin-V(膜联蛋白-V)是一种分子量为35-36KD的Ca2+ 依赖性磷脂结合蛋白,能与PS高亲和力结合。用荧光素(FITC、Alexa Fluor488等)标记的 Annexin-V 作为探针,利用流式细胞仪或荧光显微镜可检测细胞凋亡的发 … md prescriptives essentials 5 in 1WebTransfer 100 µL of cell suspension in 5 ml test tube. 3. Add 5 µL of FITC Annexin V. 4. Add 10 µL of PI solution (Cat. No. 421301) or 7-AAD (Cat. No. 420403/420404). 5. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark. 6. Add 400 µL of Annexin V Binding Buffer (Cat. No. 422201) to each tube. md prevailing wage loginWebStaining Procedure: 1. Wash cells twice with cold Cell Staining Buffer, and then resuspend cells in Annexin V Binding Buffer at a concentration of 0.25-1.0 x 10 7 cells/ml. 2. Transfer 100 microL of cell suspension in a 5 ml test tube. 3. Add 5 microL of FITC Annexin V. 4. Add 10 microL of Propidium Iodide Solution. md preliminary hearingWeb将Annexin V进行FITC标记,以标记了的Annexin V作为荧光探针,利用流式细胞仪可检测细胞凋亡的发生。 碘化丙啶(Propidium Iodide , PI)是一种核酸染料,它不能透过完整的 … mdpr health canadaWeb含 Annexin V FITC 和 PI 的死细胞凋亡试剂盒 含 Annexin V FITC 和 PI 的死细胞凋亡试剂盒可使用偶联绿色荧光 FITC 染料的重组膜联蛋白 V 检测凋亡细胞的 PS 外化,死细胞 … md preventive maintenance handbook