Caski xenograft
WebApr 13, 2024 · The CaSki and SiHa cells were cultured in high glucose DMEM (Life Technologies, Shanghai, China), the C33A and HeLa cells were grown in RPMI-1640 (Life Technologies, Shanghai, China), both medium were supplied with 10% fetal bovine serum (FBS, Gibco, USA) and 1% ampicillin and streptomycin (100 units/mL, Life Technologies, … WebAug 1, 2016 · Establishment of xenograft tumor models The mouse experiments were conducted in the Animal Laboratory Center. CaSki cells (1 × 10 7 cells) were suspended in 100 μl serum free medium and injected subcutaneously into the left flank of 4- to 6-week old male BALB/c nu/nu nude mice. Tumor size was measured with digital caliper and …
Caski xenograft
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WebAug 1, 2024 · (C) The survival analysis of established CasKi xenografts in Rag1 mice after 16E7 targeted treatment, control (nonspecific gRNA + WT Cas9), or untreated (PBS only) in days (D) The apoptotic cell ... WebMar 2, 2024 · SiHa, HeLa and CaSki cells were seeded in 24-well plates in the absence or presence of metformin and nelfinavir, alone or in combination, and the wound was generated by scratching the surface of the plates with a 10 μl pipette tip. ... we established a xenograft model in BALB/c nude female mice injected with SiHa cells and then treated with ...
WebAs shown in Figure 6D, the upregulation of DKK3, cleaved caspase-3 and E-cadherin was confirmed in CaSki xenograft-bearing nude mice treated with ApoG2, when compared with those treated with controls. Based on these results, the ApoG2 treatment was demonstrated to suppress tumor growth in CC xenografts. WebFinally, we examined the efficacy of HPV16 E6/E7 siRNA and low-dose CDDP in the HPV16-positive SiHa and CaSki human tumor xenograft models. In a previous study designed to screen various siRNA oligonucleotides, 9 siRNA 497 is one of them with the highest RNAi activity toward both E6 and E7 expression. In addition, siRNA 497 induced specific and ...
WebApr 17, 2024 · Depletion of NRP-1 significantly suppressed the growth of CaSki xenograft tumor in nude mice. Conclusions: The current study demonstrated that NRP-1 expression is significantly correlated with the progression of CC. Notably, high NRP-1 expression is correlated with a poorer survival in patients with CC, and has been shown to be an … WebIn CaSki-xenografted nude mice treated with CKD-602, a significant reduction of tumour volume was observed 94 . Namitecan (7- (2-aminoethoxy)iminomethyl-camptothecin; …
WebFeb 12, 2024 · Human cervical cancer Caski and promonocytic THP1 cell lines were cultured in RPMI 1640 containing 10% FBS, 2 mM glutamine and 1% P/S. All cells were … basa kuat basa lemahWebApr 4, 2024 · It is based on the administration of T cells that are genetically engineered to express a chimeric antigen receptor (CAR) or T cell receptor (TCR) that targets a tumor … basa kuat apa ajaWebSep 14, 2024 · CaSki (HPV16 + cervical cancer), C33A (HPV − cervical cancer, with mutations in p53 and pRb; ref. 20 ), and HaCaT (HPV − cervical keratinocytes) cell lines … sv god\u0027s-pennyWebDec 13, 2011 · Figure 6. siRNA suppression of GILT in CaSki cells shows that the abundance of GILT correlates with the abundance of the E7 11–19 peptide. siRNA suppression of GILT protein is correlated with a ... and Kreider, J. W. (1990). Infectious cycle of human papillomavirus type 11 in human foreskin xenografts in nude mice. J. Virol. 64, … basa kuat asam lemahWebApr 17, 2024 · Tumor xenograft model The CaSki cells were collected to construct a single-cell suspension. PBS was mixed with Matrigel (1: 1) to re-suspend the cells and make the … basa kuat dan asam kuatWebOct 1, 2010 · Case Series. Five Japanese girls, 1–4 years of age, presented for medical assessment with histories of developmental retardation, microcephaly, and characteristic … basakwerd putterWebNude mouse xenograft model was undertaken with HeLa cells and the xenograft tumor tissue samples were analyzed for the expression of PCNA and Ki‑67 by immunohistochemistry and the cell morphology was evaluated by hematoxylin and eosin (H&E). Results revealed that hnRNP A2/B1 was successfully silenced in HeLa and … basa kuat dan basa lemah